13C metabolic flux analysis in cell line and bioprocess development

With an ever-increasing demand for biopharmaceuticals, there is a growing need high-producing industrial cell lines. 13C metabolic flux analysis provides comprehensive perspective of host metabolism by rigorously quantifying fluxes within intracellular networks. The characterization production lines altered via engineering or media optimization can help to identify desirable phenotypes. Coupled with the advent high-throughput mini bioreactor systems and expanded modeling capabilities, provide even more complete picture metabolism. resulting knowledge cellular traits regulatory nodes offers new opportunities systematic identification targets develop optimized bioprocesses.